caspase 3 7 reporter system Search Results


caspase 3/7-luciferase assay kit  (Promega)
90
Promega caspase 3/7-luciferase assay kit
( a , b <t>)</t> <t>Apoptosis,</t> as measured by cleaved PARP and <t>caspase-3,</t> 48 h after ectopic expression of miR-103 ( a ) or ( b ) a caspase Glo- luminescence assay for active caspases 3 and 7 after ectopic expression of miR-103 in HUVECs 48 h after exposure to the indicated doses of radiation. One out of three independent experiments. Bars show mean+s.e.m. ** indicates P <0.01 by two-tailed Student's t -test. ( c ) 3D angiogenic sprouting assay with expression of miR-103. Right panel bars show mean+s.e.m. of lectin stained sprout areas from at least 10 beads per group. * P <0.05 by two-tailed Student's t -test. Scale bar, 100 μm. One of four independent experiments. ( d ) 3D angiogenic sprouting assay with expression of Anti-miR-103. Right panel bars show mean+s.e.m. of lectin stained sprout areas from at least 10 beads per group. * P <0.05 by two-tailed Student's t -test. Scale bar, 100 μm. One of three independent experiments. ( e ) Quantification of angiogenesis in mouse Matrigel plugs after i.v injection of control or miR-103 inhibitor followed by 10 Gy local radiation ( N =4–6 plugs per group, one out of the three independent experiments). Bars show mean+s.e.m. * indicates P <0.05 by two-tailed Student's t -test. ( f ) Whole mount images of mouse neonatal P12 retina stained with anti-CD31 5d post intraocular injection with miR-103. Right panel bars show mean+s.e.m. of CD31 area from n =5 retinas. * P <0.05 by two-tailed Student's t -test. Scale bar, 200 μm.
Caspase 3/7 Luciferase Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caspase 3/7-luciferase assay kit/product/Promega
Average 90 stars, based on 1 article reviews
caspase 3/7-luciferase assay kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
caspase 3/7 reporter kit  (Promega)
90
Promega caspase 3/7 reporter kit
( a , b <t>)</t> <t>Apoptosis,</t> as measured by cleaved PARP and <t>caspase-3,</t> 48 h after ectopic expression of miR-103 ( a ) or ( b ) a caspase Glo- luminescence assay for active caspases 3 and 7 after ectopic expression of miR-103 in HUVECs 48 h after exposure to the indicated doses of radiation. One out of three independent experiments. Bars show mean+s.e.m. ** indicates P <0.01 by two-tailed Student's t -test. ( c ) 3D angiogenic sprouting assay with expression of miR-103. Right panel bars show mean+s.e.m. of lectin stained sprout areas from at least 10 beads per group. * P <0.05 by two-tailed Student's t -test. Scale bar, 100 μm. One of four independent experiments. ( d ) 3D angiogenic sprouting assay with expression of Anti-miR-103. Right panel bars show mean+s.e.m. of lectin stained sprout areas from at least 10 beads per group. * P <0.05 by two-tailed Student's t -test. Scale bar, 100 μm. One of three independent experiments. ( e ) Quantification of angiogenesis in mouse Matrigel plugs after i.v injection of control or miR-103 inhibitor followed by 10 Gy local radiation ( N =4–6 plugs per group, one out of the three independent experiments). Bars show mean+s.e.m. * indicates P <0.05 by two-tailed Student's t -test. ( f ) Whole mount images of mouse neonatal P12 retina stained with anti-CD31 5d post intraocular injection with miR-103. Right panel bars show mean+s.e.m. of CD31 area from n =5 retinas. * P <0.05 by two-tailed Student's t -test. Scale bar, 200 μm.
Caspase 3/7 Reporter Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caspase 3/7 reporter kit/product/Promega
Average 90 stars, based on 1 article reviews
caspase 3/7 reporter kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


( a , b ) Apoptosis, as measured by cleaved PARP and caspase-3, 48 h after ectopic expression of miR-103 ( a ) or ( b ) a caspase Glo- luminescence assay for active caspases 3 and 7 after ectopic expression of miR-103 in HUVECs 48 h after exposure to the indicated doses of radiation. One out of three independent experiments. Bars show mean+s.e.m. ** indicates P <0.01 by two-tailed Student's t -test. ( c ) 3D angiogenic sprouting assay with expression of miR-103. Right panel bars show mean+s.e.m. of lectin stained sprout areas from at least 10 beads per group. * P <0.05 by two-tailed Student's t -test. Scale bar, 100 μm. One of four independent experiments. ( d ) 3D angiogenic sprouting assay with expression of Anti-miR-103. Right panel bars show mean+s.e.m. of lectin stained sprout areas from at least 10 beads per group. * P <0.05 by two-tailed Student's t -test. Scale bar, 100 μm. One of three independent experiments. ( e ) Quantification of angiogenesis in mouse Matrigel plugs after i.v injection of control or miR-103 inhibitor followed by 10 Gy local radiation ( N =4–6 plugs per group, one out of the three independent experiments). Bars show mean+s.e.m. * indicates P <0.05 by two-tailed Student's t -test. ( f ) Whole mount images of mouse neonatal P12 retina stained with anti-CD31 5d post intraocular injection with miR-103. Right panel bars show mean+s.e.m. of CD31 area from n =5 retinas. * P <0.05 by two-tailed Student's t -test. Scale bar, 200 μm.

Journal: Nature Communications

Article Title: MicroRNA regulation of endothelial TREX1 reprograms the tumour microenvironment

doi: 10.1038/ncomms13597

Figure Lengend Snippet: ( a , b ) Apoptosis, as measured by cleaved PARP and caspase-3, 48 h after ectopic expression of miR-103 ( a ) or ( b ) a caspase Glo- luminescence assay for active caspases 3 and 7 after ectopic expression of miR-103 in HUVECs 48 h after exposure to the indicated doses of radiation. One out of three independent experiments. Bars show mean+s.e.m. ** indicates P <0.01 by two-tailed Student's t -test. ( c ) 3D angiogenic sprouting assay with expression of miR-103. Right panel bars show mean+s.e.m. of lectin stained sprout areas from at least 10 beads per group. * P <0.05 by two-tailed Student's t -test. Scale bar, 100 μm. One of four independent experiments. ( d ) 3D angiogenic sprouting assay with expression of Anti-miR-103. Right panel bars show mean+s.e.m. of lectin stained sprout areas from at least 10 beads per group. * P <0.05 by two-tailed Student's t -test. Scale bar, 100 μm. One of three independent experiments. ( e ) Quantification of angiogenesis in mouse Matrigel plugs after i.v injection of control or miR-103 inhibitor followed by 10 Gy local radiation ( N =4–6 plugs per group, one out of the three independent experiments). Bars show mean+s.e.m. * indicates P <0.05 by two-tailed Student's t -test. ( f ) Whole mount images of mouse neonatal P12 retina stained with anti-CD31 5d post intraocular injection with miR-103. Right panel bars show mean+s.e.m. of CD31 area from n =5 retinas. * P <0.05 by two-tailed Student's t -test. Scale bar, 200 μm.

Article Snippet: Apoptosis was measured by either a caspase 3/7-luciferase assay kit (Promega) or flow cytometry with anti-cleaved caspase-3 (Cell Signaling Technologies 9579S: 1:200) and anti-cleaved PARP antibodies (Cell Signaling Technologies 5625: 1:200) or western blot for cleaved caspase-3 (Abcam Ab136812 1:300) according to manufacturer's instructions.

Techniques: Expressing, Luminescence Assay, Two Tailed Test, Staining, Injection, Control

( a ) Mir-TRAP assay depicting fold enrichment of miR-103 targets 24 h after miR-103 transfection and pull down of RISC relative to control miR. One out of three independent experiments ( b ) qRT-PCR (24 h) and ( c ) western blot (48 h) from HUVECs transfected with miR-103 showing levels of TREX1 and FANCF. Right panel shows quantitation of blots from two independent experiments. * P <0.05 by two-tailed Student's t -test. ( d ) Luminescence from 3′-UTR-luciferase constructs with indicated miR-103 binding regions 24 h after transfection with miR-103. * P <0.05 by two-tailed Student's t -test. One out of two independent experiments. ( e ) Apoptosis as measured by cleaved PARP and caspase-3 48 h after knockdown of FANCF, TREX1. One of four independent experiments. ( f ) 3D angiogenic sprouting assay after knockdown of FANCF, TREX1. * P <0.05 by two-tailed Student's t -test. One out of three independent experiments. * P <0.05 by two-tailed Student's t -test. ( g ) 3D sprouting assay using HUVECs transfected with indicated oligos. All bars show mean+s.e.m. * P <0.05, ** P <0.01 by ANOVA. 3D, three-dimensional; ANOVA, analysis of variance; qRT-PCR, quantitative real-time PCR.

Journal: Nature Communications

Article Title: MicroRNA regulation of endothelial TREX1 reprograms the tumour microenvironment

doi: 10.1038/ncomms13597

Figure Lengend Snippet: ( a ) Mir-TRAP assay depicting fold enrichment of miR-103 targets 24 h after miR-103 transfection and pull down of RISC relative to control miR. One out of three independent experiments ( b ) qRT-PCR (24 h) and ( c ) western blot (48 h) from HUVECs transfected with miR-103 showing levels of TREX1 and FANCF. Right panel shows quantitation of blots from two independent experiments. * P <0.05 by two-tailed Student's t -test. ( d ) Luminescence from 3′-UTR-luciferase constructs with indicated miR-103 binding regions 24 h after transfection with miR-103. * P <0.05 by two-tailed Student's t -test. One out of two independent experiments. ( e ) Apoptosis as measured by cleaved PARP and caspase-3 48 h after knockdown of FANCF, TREX1. One of four independent experiments. ( f ) 3D angiogenic sprouting assay after knockdown of FANCF, TREX1. * P <0.05 by two-tailed Student's t -test. One out of three independent experiments. * P <0.05 by two-tailed Student's t -test. ( g ) 3D sprouting assay using HUVECs transfected with indicated oligos. All bars show mean+s.e.m. * P <0.05, ** P <0.01 by ANOVA. 3D, three-dimensional; ANOVA, analysis of variance; qRT-PCR, quantitative real-time PCR.

Article Snippet: Apoptosis was measured by either a caspase 3/7-luciferase assay kit (Promega) or flow cytometry with anti-cleaved caspase-3 (Cell Signaling Technologies 9579S: 1:200) and anti-cleaved PARP antibodies (Cell Signaling Technologies 5625: 1:200) or western blot for cleaved caspase-3 (Abcam Ab136812 1:300) according to manufacturer's instructions.

Techniques: TRAP Assay, Transfection, Control, Quantitative RT-PCR, Western Blot, Quantitation Assay, Two Tailed Test, Luciferase, Construct, Binding Assay, Knockdown, Real-time Polymerase Chain Reaction